Product Name	Elk1 (phospho Ser389) Antibody
Antibody Type	Primary Antibodies
Product description	Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene1) Janknecht R, et al. (1993) EMBO J. 12 (13): 5097-5104. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964. More
Immunogen	Elk1 (Phospho-Ser389) antibody was raised against a peptide sequence around phosphorylation site of serine 389 (P-R-S (p) -P-A) derived from Human Elk-1.

Clonality	Polyclonal
Host Species	Rabbit
Tested Applications	IHCWB
	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	ELK1
Alternative Names	SUI1 ELK1 ETS-domain protein Elk-1
Molecular Weight(MW)	62 kDa
Tissue Specificity	This antibody detects endogenous level of Elk1 only when phosphorylated at serine 389.

Entrez Gene	2002
Protein Accession	NP_001107595.1

Application

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Phospho-Ser389).
Application

Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using Elk1 (Phospho-Ser389).

Application Notes	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Form	Liquid
Storage Instructions	Store antibody at -20˚C for up to one year.
Storage Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.